Publication in Nature Biotechnology: Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies

Founders Daryl Gohl, Dan Knights, and Kenneth Beckman collaborated with multiple colleagues in the microbiome field on an interdisciplinary project advancing the accuracy of microbiome studies. Their work was published in Nature Biotechnology.

These experiments, conducted through the University of Minnesota Genomics Center (UMGC), examined sources of error and bias in amplicon-based microbiome studies. Through molecular optimizations, the authors were able to significantly improve quantitative accuracy in comparison with other commonly used methods in the field .

In addition, Gohl and colleagues uncovered the novel phenomenon of primer editing, which they demonstrated can be used to improve the qualitative accuracy of PCR-based microbiome profiling by minimzing the drop-out of taxa with mismatches to the amplification primers.